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KMID : 1161420100130030657
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Journal of Medicinal Food
2010 Volume.13 No. 3 p.657 ~ p.664
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Isoflavone Composition, Total Polyphenolic Content, and Antioxidant Activity in Soybeans of Different Origin
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Tepavcevic Vesna
Atanackovic Milica
Miladinovic Jegor
Malencic Djordje
Popovic Jovan
Cvejic Jelena
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Abstract
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Twenty soybean cultivars, originating from the United States, Russia, Serbia, and China, were analyzed for their isoflavone composition, total polyphenolic content, and antioxidant activity. Isoflavones were extracted by aqueous methanol (80%) and analyzed by high-performance liquid chromatography/diode array detection. Precision and linearity of the applied method were within the standard limits of validation. The highest and the lowest total isoflavone contents were 4.59 and 1.45?mg/g of dried soybean weight, respectively. A significant difference (P?
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KEYWORD
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2,2-diphenyl-1-picrylhydrazyl assay, high-performance liquid chromatography analysis, soybean phytoestrogens
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